There is a positive correlation between the percentage of morphologically normal sperm and fertility. Papanicolaou staining allows identification of the acrosome and post-acrosome regions within the sperm head, cytoplasmic droplets, midpiece and tail WHO The analyses carried out in another laboratory revealed discrepancies in sperm morphology results between the DiffQuik and Papanicolaou stains Henkel et al. A relationship between the shape of the head and motility of the sperm can be sought; we should study whether the sperm with a more oval head also has a longer midpiece, organelles of which are important for the sperm movement Gage ; Gage and Freckleton The results of observations of human spermatozoa correspond with the data revealed in animal studies. Sperm morphology categorization according to Tygerberg strict criteria is also used in assisted reproduction technologies, identification of biomarkers of sperm dysfunction, and predicting male fertility potential. SB is a quick and easy two-step procedure, comparable in efficacy to the Papanicolaou technique.
The entire procedure is known as Pap smear. Both progressive and regressive nuclear staining techniques can be used in Papanicolaou stain. Georgios Papanikolaou Harald zur Hausen. The polychromatic PAP stain involves five dyes in three solutions. In sert it for 45 seconds in the dye solution 2. This method is used to differentiate cells in the smear preparation of various gynecological specimens pap smears , materials containing exfoliative cells and material from fine needle aspiration.
RAPID PAPANICOLAU||Staining kit - PROiSER R+D S.L.
Mixtures of the same name from different vendors therefore can differ in composition, occasionally producing different or poor results. The descriptions of the compositions of the staining solutions vary by source and differ even in Papanicolaou's own publications. The intensity of the nuclear staining is controlled by the immersion of the slide into a blueing agent. This method is used to differentiate cells in the smear preparation of various gynecological specimens pap smears , materials containing exfoliative cells and material from fine needle aspiration. When using the regressive staining method, the nucleus is deliberately over-stained with a non-acidified haematoxylin.
Papanicolaou (PAP) Staining : Introduction, Principle, Procedure and Interpretation
Description: Procedure 1 Standard Method: In the progressive method, the nucleus is stained with hematoxylin to a intensity desired. Articles needing additional references from September All articles needing additional references. Unsourced material may be challenged and removed.